Friday, February 4, 2011

Creating GMO's

Genetically modified organisms are organisms that have had their DNA modified by scientists to express a gene that will genetically enhance the organism.  They are intended to help the organism function more efficiently or to express a gene that will make them succeed in their environment at a greater success rate than an organism from the same species without the genetically enhanced DNA.  These GMO's are controversial globally.  They are controversial because some people believe that tampering with evolution and producing organisms that do not have 100% their own DNA is ethically wrong.  But the benefits are clearly helpful to the entire world's population.  GM food is a major source of beneficial growth among the world's supply of food. GM food is produced in order to create food that can resist frost or to kill insects that cause them to die.  GM food can also be modified to be enlarged and to alter their appearance to make them more likely to be bought at the store.  People in the United States do not have regulations on whether or not GM food has to have a label, but in other parts of the world GM food is labeled so the sales are less high in other parts of the world. 
In our lab we will use the mortar and pestil to grind up different foods to break down the nuclear membrane releasing DNA into the cytoplasm so we can extract it.  But first because DNAse is in the cytoplasm and will destroy the DNA when it contacts it we must destroy the DNAse.  We will do this using instagene matrix beads which disarm the DNAse.  Then we will take our gene of interest and place it in a TI plasmid, which we will insert into a bacteria and allow it to grow.